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To identify desirable lines, additional screens are usually performed after outgrowth, e.g. for att-site platform integrity, copy number, integration locus, etc.
Green's functions of the F-net 1D model were evaluated via a computer program for wave number integration provided by Herrmann (2013), which can be conducted on desktop personal computers.
Transduction at MOI 1 resulted in low copy number integration and transduction efficiencies below 40% (Fig. S1A C).
These findings suggest low copy number integration for the two transgenes.
Transgenic mice ('BEG') were generated and selected for copy number integration.
Copy number, integration sites, and mosaicism can vary in injected sticklebacks, giving rise to a range of expression levels.
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The results (Fig. 2) show that we were successful in obtaining cells lines with low copy number integrations.
To obtain integrated sequence arrangements in the untargeted clones as similar as possible to that of the E1 gene cluster in the adenovirus genome, we sought conditions for DNA introduction predicted to favor low copy number integrations.
Previous molecular analyses conducted on soybean hairy roots have revealed the presence of high copy number integrations [ 35, 36, 38], although the relationship between high copy number insertions and gene expression in hairy roots has not been reported.
This method utilized high-copy-number integration of myostatin gene into the ribosomal DNA of Saccharomyces cerevisiae.
The number of integration regions for the ith integration variable is given by int n_part[i].
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