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However, expression of lagC in a GBF-independent manner in a gbfA− null strain did not result in expression of the post-aggregation genes.
Furthermore, these results were consistent between two different normal mouse strains, but swelling was significantly greater in a matrix metalloproteinase (MMP -9 null strain.
Previous protein analysis suggested that the master transcriptional regulator, Spo0A, was required for their accumulation, as ChW14 and ChW16/17 proteins were absent in the spo0A null strain, SKO1.
These were transformed and screened for restoration of viability to the tpk null strain using standard plasmid shuffling.
Homozygous Dcas1/Dcas1 mutants produce fertile progeny and can be maintained as a stable Dcas null strain.
Moreover, mutational analysis using human eIF5A to complement an eIF5A null strain of S. cerevisiae supports a role for eIF5A in protein synthesis [3], [13].
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The kaiABC-null strain was created by replacement of the kaiABC DNA region with a kanamycin resistance gene [2].
The ΔgerP-null strain, however, exhibited a germination defect in this solution with only a 30% loss of heat resistant spores after 30 minutes (Figure 5).
Infection with either of the strains released only basal levels of hemoglobin, similar to the pcrV-null strain at a 1 h incubation time (gray bars, Fig. 7B).
Additionally, the ΔgerP-null strain exhibited no attenuation compared to wild-type spores, when inoculated intratracheally in our DBA/2J mouse model (data not shown).
All single deletion mutants exhibited a germination defect similar to the ΔgerP-null strain, with the exception of the ΔgerPF strain, which behaved much more like wild-type (Tables 2 and 3).
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