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b Growth phenotypes of WT, null lines, CKX2-overexpression heterozygous and homozygous lines.
The homozygous plants showed low tiller numbers (7 11 tillers/plant), as compared to null lines (18 20) and WT (17 22 tillers/plant) (Fig. 6b).
Among the T1 plants analyzed, two homozygous, 2 heterozygous and 2 null lines were selected for growth analysis in the greenhouse.
Total RNA was isolated from the shoot apex of wild type (WT) and null lines (1 4 1, 1 18 1), T2 CKX2-overexpression heterozygous (1 28 16, 1 41 6) and homozygous (1 6 2, 1 9 5) lines.
Consistent with this hypothesis, agronomic trait analysis showed insertional activation or overexpression of OsCKX2 led to reduced panicle number and total biomass in the heterozygous and homozygous plants, compared to WT and null lines (Fig. 6c, d).
a Total RNA was isolated from the leaves of wild type (WT), null lines (1 4, 1 18), CKX2-overexpression heterozygous (1 28, 1 41) and homozygous (1 6, 1 9) lines.
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The Lfng null line was obtained from Randy Johnson [17] and the Hes7 null line from Ryoichiro Kageyama [39].
Although Akt pathway activation was seen in all paediatric cells, this was considerably lower than that observed in our panel of adult glioblastoma lines, except in the PTEN null line Res186, which showed high levels of phospho-Akt.
Further detailed phenotyping was performed (supplementary methods S1) to address the in vivo function of HDAC4 which had been precluded previously due to the early mortality of the complete null line [6].
A heterozygous clone of L. donovani GLOI :: HYG (+/h) was then subjected to a second round of transfection with linearised pX63-NEO-ΔGLOI to obtain null line ΔGLOI.
cut-Psn flies were then crossed at 29°C to flies bearing either a P-element insertion in the Cam gene (Cam3909 characterized elsewhere as a recessive hypomorph) [29], [30] or a Cam null line (Camn339) [31].
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