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When the nucleus tissue is forced from the center of the disk, it can become herniated.
Total RNA (1 µg) isolated (Trizol Reagent, Invitrogen) from fresh arcuate nucleus tissue was converted to cDNA and used to screen expression levels of Sirt1, POMC, AgRP, and tubulin (internal control).
For the detection of the nucleus, tissue sections were incubated with fluorochrome 4'-6-diamidino-2-phenylindole for one minute and rinsed with PBS.
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Nucleus pulposus tissue (or herniated disc tissue) was surgically removed and homogenized.
Nucleus pulposus tissue (or herniated disc tissue) was surgically removed and homogenized; protein levels were quantified using an enzyme-linked immunosorbent assay (ELISA) for NGF.
Decellularization of healthy nucleus pulposus tissue has the potential to serve as an ideal matrix for tissue engineering of the disc because of the presence of native disc proteins and CSPGs.
However, there has been limited work characterising the time-dependent mechanical behaviour of hydrogel scaffolds for nucleus pulposus tissue engineering.
This study investigates the potential of a carrageenan gel-infused polycaprolactone scaffold for nucleus pulposus tissue engineering.
In contrast, no vector dependent activity was observed in the caudate nucleus, a tissue with no endogenous expression of PR, despite polymerase chain reaction evidence demonstrating the presence of the vector in this tissue.
Radiculopathy is dependent on both pressure and chemical substances predominantly induced by contact with nucleus pulposus tissue [ 36].
The obtained nucleus pulposus tissue was digested in a mixture of 0.01% trypsin and allowed to digest at 37°C for 15 minutes.
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