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In order to demonstrate the flexibility of our method it was decided to perform OED for a quantity that depends on the model predictions in a highly non-linear fashion, namely the time to peak for the concentration of dimerized STAT in the nucleus (state 4).
The third parameter that could not be constrained from the state variable data was v dPCN (maximum rate of degradation of nuclear phosphorylated Per-Cry complex), which was only involved in the differential equation describing the dynamics of the concentration of the phosphorylated Per-Cry complex in the nucleus (state variable PC NP ).
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Amplification was defined as more than four signals per nucleus, as stated in the manufacturer's guidelines.
In the quantum-mechanical model, a bound electron can only occupy a set of states centered on the nucleus, and each state corresponds to a specific energy level; see time-independent Schrödinger equation for theoretical explanation.
Since these systems invloves light exotic nuclei, breakup states channel play an important role that should be considered in the calculations.
Thus, like EGFP-LC3, endogenous LC3 also appears to reside within the nucleus under steady state conditions.
However, at least two RhoA-specific GE Fs Net1 and Ect2, have been shown to localize preferentially within the nucleus at steady state [8], [9].
We also demonstrate that a fraction of the total RhoA pool localizes to the nucleus at steady state, and its activity is controlled by Net1.
The finding that EGFP-LC3 and tfLC3 are enriched in the nucleus under steady state conditions additionally raises the question of whether their nuclear-cytoplasmic distribution is physiologically regulated.
This implies that the same mechanisms that retard the lateral mobility of EGFP-LC3 in the nucleus under steady state conditions are preserved and/or even further upregulated when autophagy is induced by amino acid starvation.
Immunohistochemistry showed pS6 signaling is increased in AgRP FoxO1 KO neurons projecting from the arcuate nucleus, signaling a state of abundant nutrients.
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