Exact(3)
The distal tip of ND did not extend to the level of the oculomotor nucleus (section M).
Then the nucleus section was collected and incubated with buffer B. The product was then digested with micrococcal nuclease, which finally stopped with 0.5 M EDTA on ice.
Originating from MT organizing centers, MTs are responsible for the correct positioning of cellular organelles, the mitotic spindle during cell division [ 213], and the nucleus (Section 3.2).
Similar(57)
The FR penetrates and contacts the dorsomedial part of the red nucleus (sections 2 4 in Fig. 10).
The distal tip of NB exits dorsally to the rostral part of the oculomotor nucleus (sections N P).
To verify the TUNEL staining was localized in the nucleus, sections were counterstained with 4,6-diamidino-2-phenylindole (DAPI) after the reaction.
Lentiform nucleus sections from normal controls showed minimal TMEM106B expression; additionally, neurons of the nucleus basalis of Meynert had little to no staining (not pictured).
To investigate which cell-specific makers co-localized with BrdU-positive nucleus, sections were treated with different antibodies including vWF (Abcam), nestin (Abcam), and coimmunostaining with BrdU.
Given the putative role of TMEM106B in FTLD-TDP, we stained frontal cortex, occipital cortex, cerebellar, hippocampal, and lentiform nucleus sections from individuals with GRN FLTD-TDP, GRN FTLD-TDP, and normal controls.
This is especially visible on the intensity profiles where the signals for PADI6 and MATER between the two nuclei (section iii) are almost absent and much more elevated in the cortex (sections i & v) for the O/N 4°C condition.
To stain cell nuclei, sections were incubated in a solution containing DAPI for 1 min.
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