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Estimates of the number of homeodomain protein molecules per nucleus range from 20,000 to 50,000 [ 18– 218.
Estimates for cyanobacterial genes in the nucleus range from 6% in the green alga Chlamydomonas reinhardtii (Moustafa and Bhattacharya 2008), to about 11% in the glaucophyte Cyanophora paradoxa (Reyes-Prieto et al. 2006), and to as high as 18% in A. thaliana (Martin et al. 2002).
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From these data it is evident that a subset of RhoA-associated proteins are present in the nucleus, ranging from GEFs (Net1, Ect2), GAPs (DLC1, p190 RhoGAP) and GTPases (RhoA, RhoC, Rac1), to downstream effector proteins (ROCK II, LIMK).
An immunohistochemical grading scale for α-enolase expression into the cytoplasm and nucleus, ranging from none (0) to weak (1), or moderate (2) to strong (3), was empirically determined.
The percentage of cells (n≥200) with strong punctate or patchy staining ranged from 35% (pSuper, NT) to 67% (shRNA1); whereas, those cells with a more diffuse or relatively homogenously BrdU staining (containing discrete foci of BrdU immunoreactivity equally distributed throughout the nucleus) ranged from 65% in controls to 32% in knockdown experiments (Figure 3C).
Mean ERCC4 signals per nucleus ranged from 1.37 to 2.35 (median = 1.82), whereas CEN-16 ranged from 1.40 to 2.06 with a median of 1.68.
The authors reported that the EXP protein itself forms nuclear foci in MyoD-transfected DM1 fibroblasts, and that their number per nucleus ranged from 6 to 30, with an average of 15 foci (Supplementary Material, Table S1).
A median of 1024 high-quality nuclei (range: 204 1744) was measured per case.
In a pilot study, Stefan et al. demonstrated in binarized chromatin images of H&E-stained cytologic smears a higher FD for dog lymphoma nuclei (range 1.588 1.617) than for normal lymphoblasts (range 1.503 1.536 [143]), but since these results are based on very few cases, these data should be interpreted with caution.
While the average nuclear number is indicative of overall fusion in an embryo, we also chose to analyse these mutants by calculating the percentage of hemisegments that showed a specific number of nuclei ranging from 1 5.
p21 was seen in 82 (90%) infiltrating tumours; staining was heterogeneous; the percentage of reactive nuclei ranged from 1%to35%5%.
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