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The extensive analysis of nucleus position patterns revealed a (nearly) tetrahedral symmetry of cell distribution (Fig. 5 a d).
Less frequently, we observed two bacteria that were symmetrically distributed from each other, considering the nucleus position (Fig. 2A-c).
Migration distance analysis was performed by averaging the cell nucleus position measured at the farthest distance from the edge of the explant at 4 points around the explant.
With χ = 2 for the sphere, the nucleus position pattern of spherical egg should contain at least 12 pentavalent positions (in the case of N7 = 0).
The analytical form of the tetrahedral nucleus position pattern was obtained by imposing close packing conditions to a tetrahedral distribution obtained numerically.
A space-time plot was generated from this data showing nucleus position as a function of egg length over time (0% egg length corresponds to the anterior pole and 100% egg length corresponds to the posterior pole), where white indicates presence of a nucleus (high fluorescence intensity) and black indicates absence of a nucleus (low fluorescence intensity) (Figure 2A).
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In order to test this possibility, we performed permutation tests through which the geometric pattern of TUNEL-positive cells was compared to random geometric patterns each generated by reallocating the positive signals to n randomly chosen positions among all observed nucleus positions (i.e. "1200"+"60") on the egg surface.
These proteins stretch out into the cytosol and are involved in the nucleus positioning.
This might reflect again a problem with the nucleus positioning machinery.
This feature for SA and SE cells could reflect a delay in anaphase onset due to abnormal nucleus positioning.
Mechanistically, the nucleus positioning machinery relies on two spindle positioning pathways before anaphase: the Kar9p pathway (Kar9p/Bim1p/Myo2p) and the dynein pathway (Dyn1p/Bik1p/Kip2p).
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