Interestingly, at the ultrastructural level we observed that both dsRNA and syncytin colocalized in the same clusters, scattered throughout the cytoplasm and nucleus, panel C).
With a false discovery rate-corrected threshold (P < 0.05), these clusters covered a large expanse of the anterior cingulate gyrus (panel 1), the head of the caudate nucleus (panel 3) and the posterolateral orbitofrontal cortex (panel 4).
These augmentations in myelination correlated with the increased number of p27KIP1 positive nuclei (panels B, E, H, and K) that was significantly higher in QKI-6/7 as compared to GFP-transduced co-cultures (Figure 4, compare panels B and K).
They were in close contact to eukaryotic cells resembling polymorphonuclear leukocytes (PMN's) according to the presence of polymorph nuclei (panel C).
The nuclei of control cells transformed with the empty vector fluoresced intensely with propidium iodide (PI, red) and exhibited only TUNEL-negative nuclei (panel empty vector).
ArhGAP44 R291M) is absent from the nucleus (bottom panels), but present at distinct patches along the dendrite (top panels, red arrow).
As shown in Figure 2, GFP alone was distributed throughout the cytoplasm and the nucleus (left panel), whereas GFP-AtGILP was exclusively localized in the plasma membrane (middle panel).
Interestingly, this staining co-localized with DAPI labeling, in the nucleus (middle panel).
Since FISH can be performed on interphase nuclei, these panels allow for target detection of specific chromosomal abnormalities even when metaphase cytogenetics is not possible because of no mitosis [ 37].
Pol κ-deficient cells showed high levels of RPA focus formation and a significant increase in the number of RPA foci per nucleus (right panel) as compared with control cells containing Pol κ.
