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The combination of sensitive quantitative fluorescent in situ hybridization (Q-FISH) and three-dimensional (3D) microscopy have evolved as powerful tools in studying the dynamic 3D organization of telomeres and chromosomes in the interphase nucleus of individual normal and tumor cells.
A region over the nucleus of individual cells was defined and the average fluorescence intensity of the region was quantified as a function of time.
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At the PTB's ion accelerators, a new microbeam facility is now in operation that is capable of delivering single ions, for example, to the nuclei of individual living cells.
Samples were analysed in a FACScan flow cytometer, measuring green (dUTP-FITC incorporated in fragmented DNA) and red (PI binding to DNA) fluorescence of nuclei of individual cells.
In the following, we first characterize the statistical properties of random variations in expression level between adjacent nuclei of individual embryos and compare them to the results of stochastic simulations.
Adjacent sections were used and counting was performed in similar areas; quantitative analysis performed with a computerised analyser system (Ibas 2000, Kontron, Zeiss) was used to score the nuclei of individual cells for expression of these proteins.
Our results demonstrate intact H2AX phosphorylation and γH2AX cluster formation in nuclei of CD8+CD28+ T cells, while CD8+CD28− T cells failed to mount such a response properly, both in terms of the percentage of cells with γH2AX clusters in their nuclei as well as the numbers of clusters in the nuclei of individual cells (Fig. 3).
Although the levels of Prm1 mRNA and Prm2 mRNA, which are expressed in round spermatids, were significantly reduced in 3-month-old Bscl2−/− testes compared with Bscl2+/+ testes, the levels of PRM1 and PRM2 proteins, which are expressed in late spermatids and sperm, were comparable in the nuclei of individual Bscl2+/+ and Bscl2−/− late spermatids and sperm (Supplementary Figure S6).
In the periodic table, the number of an element refers to the number of protons in the nucleus of an individual atom.
Mean FRET efficiencies were measured in the nucleus of 25 30 individual cells for each combination.
To study the dynamics of the pumping mechanics of Drosophila heart in live, we used a transgenic Drosophila line, Toll-nGFP, where GFP is expressed in the nucleus of all individual cardiac cells [18].
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com