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The immunofluorescence labeling of cyclin A2 showed its presence predominantly in the nucleus of control K562 cells (Fig. S4), whereas in cells administered with DOX, cyclin A2 was mainly located at the cytoplasm of early and late phases of apoptotic cells (Fig. 6B).
A high expression level of Ki-67 was also observed in the tumor cell nucleus of control mice.
While p65 was detected in the nucleus of control and TNF-α-treated cells, almost no signal for p65 was found after RML6 infection.
Taken together, these data suggest that in the nucleus of control MCF7 cells PTB is present in a complex that includes the RNA-binding proteins NONO/p54nrb, PSF, hnRNPA1, hnRNPC1/C2 and hnRNPA2/B1.
Similarly, we observed that Bax localized in the nucleus of control A549 cells; however, in IAV NP-transfected cells, a substantial amount of Bax was detected in the mitochondrial region.
As Par3 was found in the nucleus of control cells only after HGF treatment, the change in subcellular localization for Par3 and aPKC caused by Numb depletion suggests that the numb-shRNA cells already acquired certain characteristics of mesenchymal cells before HGF stimulation.
Similar(54)
3MC significantly increased the expression of AhR XRE complexes, but high-dose ST-1 decreased the expression compared with that of the hepatic nucleus of controls.
2MeH3K9 was broadly detected in nuclei of control cells including eu- and heterochromatic region (Fig. 1C).
Quantification of several hundred nuclei showed that this phenotype is predominant in activated BXB-ER nuclei and essentially absent in the nuclei of control cells including 3T3 cells treated with estrogen (Fig. 1C).
These results reflect the fact that, in Wnt5a−/− mice, the nuclei of apical FP cells are oriented ventrally, while the nuclei of control mice are oriented more ventro-laterally (Figure 7A, B).
Pinocembrin detected at the same concentrations did not damage the nuclei of control cells.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com