Since oocyte nuclei measured between 20 30 µm in diameter, counting every 5th section of the ovary ensured a distance of 40 µm between analysed sections, preventing multiple counts of the same ovarian follicle.
An average of 6 15 adjacent microscope fields were screened with a total of 50 consecutive tumour cell nuclei measured in each sample.
TUNEL analysis showed that the number of apoptotic nuclei, measured from the border zone and in the area remote to the infarct, was higher in GK rats after MI as compared to Wistar controls.
The percentage of apoptotic nuclei, measured using the TdT-mediated dUTP-biotin nick end labelling (TUNEL) method, was significantly lower in LPD-treated compared with SD-treated mdx mice, both in TA and diaphragm.
Similarly, the mean fluorescence intensity (integrated density divided by area) was plotted as well as the radius of the G1 nuclei, measured drawing a line through the nucleus at its broadest area using ImageJ, dividing it by two and plotting it against the age of the cell.
(B ) Percentage of plasmids at a resolvable distance to the nuclear periphery in wt, gcn5∆ and sus1∆ mutant cells expressing Nup170 fused to TetR (mean ± SD, N = 3 clones) (C ) Fluorescence recovery after bleaching the bud part of early anaphase nuclei measured in cells expressing several NPC components tagged with GFP.
Of note, one cell nucleus measures between 3 and 6 pixels.
Unlike hepatocytes or myocytes, each neural cell contains a single nucleus, so measuring DNA content evaluates the number of cells (Winick and Noble 1965).
These secondary beams produced at relativistic energies are used for Coulomb excitation or secondary fragmentation experiments in order to explore the nuclear structure of the projectiles or projectile like nuclei by measuring de-excitation photons.
We tried locating the locus (ratio) of unstained nuclei, and measuring the spread about this locus, to set a boundary independently for every image (tissue core).
