Exact(4)
Surprisingly, the nucleus height was substantially increased inside the microtubes, which cannot only be accounted for by the 2D confinement, as the cell nuclei dimensions were hardly affected in the microtube diameter range from 8to1717 μm.
This is in agreement with our nucleus height "H", which is a parameter slightly different than the disc height measured by Chen et al. We found a decrease of "H" with increasing the severity of spondylolisthesis.
The cell nucleus height and length remained fairly constant in this microtube diameter range, while the nucleus width slightly decreased with decreasing microtube diameter (data provided in Table S1 and depicted in Figure S1, Supporting Information).
Therefore, we fabricated different SiO/SiO2 scaffolds with microtube diameters ranging from 4 μm (the average nucleus height of free growing cells) to 25 μm (a little larger than the average nucleus length of free growing cells).
Similar(56)
As can be seen in the inset of Fig. 1b, the shadowed length is given by h tan θ for a nucleus with height h [44].
As cells were not imaged throughout their entire thickness in the z direction, we computed mRNA densities by dividing single-cell counts by the volume of the prism with base corresponding to the polygonal segmentation of a given cell's nucleus and height equal to 0.4 μm multiplied by the number of image planes minus one.
where Ω is the solid volume of the Ga-As pair (4.51 × 10−29 m3), h is the height of crystal nucleus, β is the contact angle of the droplet, γLV (γSN) is the interface energy between the droplet and the vapor phase (between the crystal nucleus and the NW top surface), and γSL,ZB(WZ) and γSV,ZB(WZ) are the interface energies of the GaAs ZB (WZ) crystal nucleus top and side surfaces.
Ossific nucleus width and height (lateral and medial thirds) was also recorded to document the progression of ossific nucleus development compared to the contralateral side.
The decrease of the nucleus width and height below a value of 6 ± 1 μm can only be compensated for by a considerable increase of the nucleus length indicating a significant remodeling of the cell nucleus content.
Any reduction of the microtube diameter below this threshold resulted in a linear and equal decrease of the nucleus width and height from 6 ± 1 to 4 ± 1 μm (see Table S1, Supporting Information) while the nucleus length increased to compensate for the restriction and to conserve the volume of the cell nucleus.
10 The four main traits were scored 0 3 as previously: disc signal intensity within the nucleus pulposus, disc height measured in the middle of the disc, lumbar disc extension into the spinal canal and anterior osteophytes using the five middle slices of the sagittal scan.
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