As with the nucleus, full-length KHC completely rescued the formation of the DAs (supplementary material Fig. S1), whereas 67%and52%2% of the KHC1-700 and KHC1-849 eggs, respectively, have no DA (Table 1).
The timing of this unique process begins with the appearance of conoids in late S phase [8] and culminates with fully formed daughters possessing a single nucleus and full complement of organelles; e.g. plastid, rhoptry, and micronemes [35], [37].
ND5c was more accumulated in the nucleus than full length NFAT5c or NFAT5a in isotonic conditions (290 310 mOsm/kg) (Figure 7B and Figure S4), which suggested that the NES and AED were not sufficient to ensure a complete cytoplasmic localization of an NFAT5c construct lacking the CTD.
However, closer examination revealed that cdc6-mn cells exhibit an increase in the number of cells with regions of the nucleus without full SC assembly, which is what we counted.
Nuclear Overhauser Enhancement NOEE) measurements revealed that less than 50% of the non-equivalent carbon nuclei experienced full NOE of 2.98 while more than 25% of the corresponding carbon nuclei showed a NOE of less than 2.75.
Results of several previous studies also demonstrated the translocation of EGFR in the nucleus as full-length receptors (Marti et al, 1991; Lin et al, 2001; Li et al, 2009).
In region 2A, up to four neighboring nuclei contain full-length SC and enter meiosis.
However, recent evidence has suggested that Sirt3 can also be present in the nucleus in its full length form (Scher et al., 2007; Iwahara et al., 2012).
ERBB family receptors contain nuclear localisation signals [ 27] enabling them to translocate to the nucleus, either as full length molecules (e.g. the EGFR) or as fragments (e.g. ERBB4) after proteolytic processing [ 28].
Similar to GLI2, full-length GLI3 can be degraded in the cytoplasm or enter the nucleus as a full-length activator (GLI3A) or cleaved repressor (GLI3R).
Both types of nuclei contain the full complement of genes that bear the hereditary information of the organism.
