Exact(9)
Svevo using a phenol/chloroform large-scale nucleus extraction protocol45.
As controls, GAPDH was only detected in cytoplasm extraction, and Lamin A/C was only present in nucleus extraction.
The other part was handled for cell nucleus extraction using a nuclear extraction kit (SN000, Solarbio, Shanghai, China).
The beads were washed with five volumes of nucleus extraction buffer with 350 mM NaCl, followed by five volumes of low-salt extraction buffer (150 mmol/l NaCl).
Nucleus extraction and staining were prepared with a CyStain UV ploidy kit (Partec CyStain UV ploidy kit, Partec GmbH, Münster, Germany).
Jiang et al. [ 3] proposed a WBC segmentation scheme on color space images using feature space clustering techniques, scale-space filtering for nucleus extraction, and watershed clustering for cytoplasm extraction.
Similar(51)
Nuclei extraction was prepared following the procedure presented in Galbraith et al. (1983) and Doležel et al. (2007 a ).
Nuclei extraction from 0.5 g of Arabidopsis inflorescences was performed according to the protocol used for ChIP experiments [ 7].
Frozen samples were first gently dounce homogenized for 2 min in 2 ml nuclei extraction buffer (NEB) with 0.1% Triton X-100.
Proteins were extracted from whole cells (RIPA lysates, protocol as described previously) [65] or from the nucleus (nuclear extraction).
To evaluate the efficacy and safety of phacoemulsification using torsional modality with different parameter settings for hard nucleus cataract extraction.
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