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Similarly, in intermediate and late spermatids where pronounced nuclear elongation and condensation took place and a microtubules complex was present in vicinity to the nucleus, expression of the protein was maintained at a high level and most protein signal was detected around the nucleus and overlapped with perinuclear microtubules.
Whereas overexpression of wild-type AKAP79 had no effect on either thapsigargin-evoked Ca2+ signals or on the ability of NFAT-GFP to migrate to the nucleus, expression of an AKAP79 mutant (ΔPIX-AKAP79) that cannot bind calcineurin [ 42] inhibited NFAT accumulation in the nucleus without altering the Ca2+ signal.
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Interestingly, in Nuc1 rat, where a mutation in the Cryba1 gene inhibits normal programmed loss of nuclei, expression of both Bit1 and PKD remains abnormally high in mature lens fibers.
Myrionecta separately sequesters the cryptophyte nucleus and expression of plastid genes from the captured nucleus and nucleomorph has been demonstrated [ 25].
These results suggest that in the nucleus the expression of VHL and TLX/HIF-α correlate negatively even though they may exist simultaneously in the nucleus.
STA-21 treatment of cells disrupted Stat3/DNA complexes, abrogated Stat3 translocation into the nucleus, inhibited expression of proteins such as Bcl-XL and Cyclin D1 and induced the apoptosis of breast cancer cell lines.
In the presence of glucose, Mig1 recruits Tup1 to its targets to repress their transcription, while in low glucose Snf1 phosphorylates Mig1, resulting in Mig1 export from the nucleus and expression of its target genes.
This increase in levels of IκBα might have effected the translocation of NFκB to nucleus, effecting expression of several genes involved in Y79 cell proliferation.
To analyze the levels of Nrf2 and small Maf protein in the nucleus, the expression of nuclear proteins were measured by western blotting.
Relocalization of the SMAD2/3/4 complex from cytoplasm into nucleus triggers expression of many genes including those linked to cell cycle arrest (for a review, see [ 106]).
In the presence of glucose, Mig1 becomes dephosphorylated and localizes to the nucleus repressing expression of target genes such as SUC2.
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