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In control group, the nucleus color of smooth muscle cells in aortic plaque was dark and CDK4 expression was strong(A)).
In C-PC treatment group and CD59 transfection group, the nucleus color of smooth muscle cells was lighter, brownish-yellow granules were less, and distribution was uniform(B,C)).
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Green, blue, and red colors correspond to the luminescence of cell cytoplasm (colored with GFP), cell nuclei (colored with Hoechst) and SiNPs, respectively.
Moreover, we used real images from histological stained tissue in which we were interested in brown (CD31/34 immunolabeling for vessels) and blue (hematoxylin for nuclei) colors.
Each nucleus is colored from light to dark for low to high probability of eve being ON: within stripes the color scale is from white to black and outside the stripes it is on a red scale, with peach for values below 0.15.
For the immunofluorescent staining, after the cells were fixed with 4% paraformaldehyde (Sigma-Aldrich) and permeabilized with 0.3% Triton X-100 (J.T. Baker), diamidino-2-phenylindole (DAPI; Sigma-Aldrich) and rhodamine phalloidin (Biological Industries) were used to stain the nuclei (blue color) and actin filaments (red color), respectively.
Cell adhesion images, using immunofluorescent staining, of HNEpC on the Ti specimens (M, E1, and E2) after 12 h of cell incubation: dual staining of DAPI for nuclei (blue color) and rhodamine phalloidin for actin filaments (red color) (arrow: spreading cells).
Haematoxylin was used as contrast staining for BCCIP negative cell nuclei (blue color).
Osteoclasts (three or more nuclei, purple color) and osteoblasts (dark blue color) were identified in the TRAP-osteocalcin double-stained slides.
As illustrated by the representative photomicrographs in Figure 7, immunofluorescence based experimentation showed that untreated LCC6 and LCC6Her2 cells contained normal intact nuclei (blue color) and typical F-actin cytoskeleton (red color) with distinct intracellular organization and prominent stress fibers.
Nuclei were colored by Hemalun.
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