Of the 99 genes, 47 belonged to the GO cellular component category "nucleus" (FDR = 3.82 × 10−6, 1.9 fold enrichment), and 32 were in the GO molecular function category "transcription factor activity" (FDR = 1.8 × 10−4, 3.5 fold enrichment).
The pKa of these compounds were also correlated to ΔMEP (i.e., the MEP evaluated for the isolated neutral acidic atom subtracted from the MEP value on the acidic nucleus for each category of compounds; Liu and Pedersen, 2009).
For each brain, the percentage of nuclei in each category was calculated.
All nuclei classified in category A were DARPP-32-immunolabeled (100±0%) and virtually no DARPP-32-positive neuron was misclassified into the other categories (1±1%).
A minimum of 100 tumor cells were scored with the percentage of tumor cell nuclei in each category recorded.
According to cellular component ontology, we first classified 1,362 orthologous quartet members into six categories (nucleus, cytoplasm, organelle, cell membrane, extracellular, and unknown) (fig. 2).
Partitioning a total of 1,362 orthologous targets into six subcellular categories (nucleus, cytoplasm, organelle, membrane, extracellular, and unknown), we applied the Ka/ Ks ratio to evaluate and compare the evolution rate of these druggable protein targets.
cER Histo (H -score = (% of positively stained tumor cell nuclei at weak intensity category × 1) + (% of positively stained tumor cell nuclei at intermediate intensity category × 2) + (% of positively stained tumor cell nuclei at strong intensity category × 3).
In the ontology cellular component all categories besides nucleus are suppressed.
On the right, the histogram shows the percentages of nuclei of each depicted category.
