TorsinA is widely expressed throughout the central nervous system in humans, but is found at particularly high levels in dopaminergic neurons of the substantia nigra, locus coeruleus, Purkinje cells, cerebellar dentate nucleus, basis pontis, thalamus, hippocampal formation, oculomotor nuclei and frontal cortex [ 13- 16].
Torsin A is expressed throughout the CNS in humans, but is found at particularly high levels in the dopaminergic neurons of substantia nigra pars compacta, locus coeruleus, Purkinje cells, cerebellar dentate nucleus, basis pontis, thalamus, hippocampal formation, oculomotor nuclei and frontal cortex (Shashidharan et al., 2000; Konakova et al., 2001; Rostasy et al., 2003).
We hypothesized that CHI has a characteristic pattern of distribution, and that infants with CHI have a significantly greater incidence of injury to pre- and post-cerebellar nuclei critical to cerebellar processing, i.e. inferior olivary nuclei, basis pontis and cerebellar dentate, compared to control infants who did not sustain CHI.
"Addressing the immediate needs of malnutrition, this is, of course, the nucleus and the basis of our response," said Dr. Wolman.
The protein shuttles between the cytoplasm and nucleus on the basis of targeting signals that are recognized by importins for nuclear localization and exportin-1 for nuclear export.
In Arabidopsis MgPPIX was hypothesized to be a retrograde signal from the chloroplast to the nucleus on the basis of data obtained with mutants that are defective in the norflurazon (NF) induced down-regulation of transcription of light harvesting complex protein B (LHCB) [gun (genomes uncoupled) phenotype].
Notably, based on dual immunohistochemistry for Hb9 expression with cadherin in situ hybridization, we were able to distinguish two subsets of the ventral hypoglossal motor nucleus on the basis of the differential expression of cadherin-13 and cadherin-6b, with cadherin-13 being predominantly expressed in a lateral grouping of the ventral hypoglossal from st29.
The DAPI beads could be separated from the nuclei on the basis of their forward versus side scatter profiles.
The Ki-67 index was defined as the proportion of MIB-1 positive tumor nuclei on the basis of a manual count of 500 cells.
The DNA ploidy status measured using image cytometry overcomes some of these disadvantages by allowing the visual control as well as the selection of nuclei on the basis of morphologically or additionally measured features, such as nuclear shape and texture.
For example, prokaryotes, which are single-celled organisms without a nucleus, form the material basis for single-celled eukaryotes, which are organisms that do have a nucleus; in turn, single-celled eukaryotes serve as the material basis for multicellular eukaryotes.
