Sentence examples for nucleotide replacement from inspiring English sources

The gene recombination was designed to generate only a nucleotide replacement without any other DNA changes such as insertion of external DNA fragments.

We report that a single nucleotide replacement in the melanocortin 1 receptor gene [1] (mc1r) is responsible for the white coat color of the "Kermode" bear [2], a color phase of the black bear (Ursus americanus Pallus) found in the rainforests along the north coast of British Columbia.

Co-transformation of the arginine-requiring mutant, arg7-8, with Cas9/intron-sgRNA constructs and appropriately designed synthetic, 80 nucleotide ssDNAs complementary to the argininosuccinate lyase (ARG) gene led to successful homologous recombination or nucleotide replacement and production of arginine prototrophs.

The nucleotide replacement happens before translation starts, and is mediated by various guide RNAs and enzymes that excise old and insert new nucleotides in a variety of ways to yield a product that is no longer complementary to the DNA stretch from which it was originally derived, and a protein that is no longer co-linear with the DNA sequence in the classical molecular biological sense.

No restoration of BM2 synthesis was observed with either of the 6.1 nucleotide replacement mutants (Figure 1B).

To confirm if versican was a target of miR-199a*, U343 cells were co-transfected with versican 3'UTR-luciferase construct (lu-VUTR) or the mutant lu-Ver-mut, in which the miR-199a* target site was mutated by nucleotide replacement (Fig 4i, upper).

These were calculated by determining the minimum number of nucleotide replacements that would result in the transformation of the cytochrome c amino acid sequence for one species into that of another.

Out of 36 sequenced genes, 8 genes showing more than 4 nucleotide replacements were selected and the corresponding sequences were used to construct unrooted trees.

Along with silent nucleotide replacements, the common nucleotide variations polymorphisms could be arranged into at least 15 coding region haplotypes as deduced by direct sequencing of PCR amplified DNA and also by cloning and sequencing of individual PCR products from certain heterozygous animals (Figures 1, 2).

Deletions, insertions and nucleotide replacements were respectively responsible for 76, 21 and 19 gene disruptions.

To prohibit allele conversion, we "locked" the MMR genes through nucleotide replacements.