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Blastn compares a nucleotide query sequence against a nucleotide database.
Comparison of blastn (search a nucleotide database using a nucleotide query) and tblastx searches (search translated nucleotide database using a translated nucleotide query).
3. BLASTN also compares nucleotide query sequences to a nucleotide database.
It is also possible to choose to run the application in BLASTN or TBLASTX mode, i.e. by searching nucleotide databases using a nucleotide query or by searching translated nucleotide databases using a translated nucleotide query, respectively.
The nucleotide query sequence is run in all six reading frames by BLASTX against all selected reference databases individually.
The workflow for the taxonomic assignment of a nucleotide query sequence comprises three stages (Fig. 1a c).
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We blasted all 454-FLX reads and composite Illumina reads against the NCBI protein database nr [21] using translated nucleotide queries (blastx parameters -b 100 -v 100 -e 30 -Q 11 -F "m S"), and used the popular metagenomics software MEGAN [22] for downstream analysis.
The program nhmmer is used to search one or more nucleotide queries against a nucleotide sequence database.
For nucleotide queries there is an initial gene prediction step (using MED-Start) to generate a proteome, although.
Two CDD search services are available: CD-Search (8), for protein and nucleotide queries and Batch CD-Search (9), for multiple protein queries.
Sequences were annotated according to homologies to protein database searches using translated nucleotide sequences and direct nucleotide queries http://blast.ncbi.nlm.nih.gov/Blast.cgi.nih.gov/Blast.cgi
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