"In each case, you either have a nucleotide profile that is favourably associated with improvements in aerobic capacity with exercise training, or not.
Some of the matches corresponded to nucleotide profiles determined from collections of phylogenetically conserved, cis-acting regulatory elements [ 36].
The variations of single nucleotide profiles and poly(A) signals are clearly different among different genic and intergenic regions.
We focussed on Rhox2A-H and Rhox4A-H and used these nucleotide profiles to determine whether these paralogues were expressed.
Among the FUE regions (around −100 to −28), G and C nucleotides had greater content than A and U nucleotides in the single nucleotide profiles.
The number of polyA sites in generated reads was counted and plotted against their distance from TSS. (B ) The nucleotide profiles of polyA sites in (A ).
Despite such a high similarity we were able to build up a unique nucleotide profile of each copy of Rhox2, 3and 4 allowing them to be definitively identified.
Eight of the 15 core and proximal promoter motifs we looked at occur differentially between FAPs and DAPs, and the DAPs have a distinct nucleotide profile.
They base this argument on their observation that 3' ends appear to be more conserved than 5' ends; this observation is supported, but only nominally, by our 3' and 5' nucleotide profiles.
Each unique nucleotide profile for Rhox2 and 4 has a minimum of 3 different nucleotides between paralogues with the exception of Rhox2B and E (2 nucleotides different), Rhox2D and G (1 nucleotide difference) and Rhox4A and C (2 differences).
