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The signal strength was assessed from the Fourier transform of the oscillating curve of the motif/complement count versus the nucleotide number.
The counts were averaged in a 60 nucleotide window that slid 5 nucleotides per step to generate a continuous curve of VWG/CWB counts as a function of nucleotide number.
The histogram data were then averaged in a sliding (5 nucleotide increments) 60 nucleotide window to generate a continuous curve of average period-10 motif/complement count versus nucleotide number.
The insertion in antisense orientation is controlled by sequencing, and the sequence of the inserted cDNA fragment (392 bp) corresponded to nucleotide number 4369 to nucleotide number 3978 (accession number: BC152454; gene id: 5738 PTGFRN).
Albeit, correlations were highest with genome size (nucleotide number) followed by number of CpGs in a given genome, followed by number of present X chromosomes.
This position corresponds to the nucleotide number 360 (where there is 'C') in the complete mitochondrial genome sequence of G. gallus (Acc. No. NC_001323) available in GenBank.
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Nucleotide numbers refer to the original sequences.
Based on a previous report, a large deleted region would seem to exist in the Hind III J fragment located between nucleotide numbers 2439 and 11,270.
Nucleotide numbering corresponds to the COL5A1 reference sequence (NM_000093.3) and is according to HGMD guidelines.
The start of nucleotide numbering was selected to correspond with the published GAS genomes.
Nucleotide numbering goes from 1858 to 3182 and restarts at 1.
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