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The nucleotide matrix is available upon request.
The concatenated nucleotide matrix was partitioned according to genes.
Bayesian and maximum parsimony analyses for the nucleotide matrix of PpDAM sequences were also conducted.
Bayesian and maximum parsimony analyses for the nucleotide matrix were also conducted.
Bayesian inference (BI) analysis was carried out in MrBayes 3.1.2 [ 32] after partitioning the nucleotide matrix according to genes.
The resulting alignment is presented in the Additional file 4. Maximum likelihood analysis using the nucleotide matrix was conducted using PAUP* 4.0b10 [ 49].
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Gap characters representing mainly deletions were studied separately from the nucleotide matrices.
Catsper1 nucleotide matrices and resulting phylogenetic trees are available in the TreeBASE repository (http://www.treebase.org) with accession URL: http://purl.org/phylo/treebase/phylows/study/TB2 S15721 [ 57].
To infer phylogenetic trees from three nucleotide matrices, we applied the maximum likelihood method with rapid bootstrapping of 1000 replicates in RAxML-HPC v7.2.8 available on http://www.phylo.org/ [ 80].
A General Time Reversible (GTR) model with a Dirichlet (flat) probability distribution of nucleotide rate change parameters, stationary nucleotide frequencies, no specified shape parameter for the gamma distribution of rate variation, and no invariable sites was used for the nucleotide analyses; this is the default prior model for nucleotide matrices in MrBayes.
Both the mono-nucleotide matrix and the di-nucleotide matrix were optimized in the same window.
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