Sentence examples for nucleotide marker from inspiring English sources

Therefore, we considered the ∆530 locus to be a nucleotide marker for non-JP2 strains and designed a qPCR primer set and hydrolysis probe specific to the non-JP2 strains in this region (Table 1).

As these markers are physically linked, separated by 600bp or less in a single contiguous sequence, we analyzed the SNPs as representing the locus rather than a particular marker and placed the locus on the linkage map rather than the individual nucleotide marker.

Recently, Correia et al. ([2011]) showed that tetra-, penta- and hexa nucleotide microsatellites were more informative than the di and tri nucleotide markers and specific SNPs employed for assessment of parentage and individual identification.

Eleven single nucleotide markers were tested for the sGBS approach.

Microsatellite and single nucleotide markers used to assess zygosity Table S3.

The final dataset consists of 207,321 single nucleotide markers (SNPs) with an average genotyping rate >99.7% in 967 individuals.

Genotype data for the parental strains and segregants were obtained (Mancera et al. 2008) and filtered to include only single nucleotide markers, which resulted in 48,934 markers.

The overall success rate of marker development, from the step of sequencing to primer design was 57.3% for the di and tri- nucleotide markers which were targeted in our study.

A potential problem arises, however, when there is a singular mutated mono-nucleotide marker.

Three microsatellite loci (Str73, SsoSL417 and SsaD71) had some missing data in the Falklands populations and one tetra-nucleotide marker (Ssa171) presented some alleles that differed only in two base pairs, probably due to the compound motif GTGA + GT.

F1-Δ rca-1, the Δ rca-1 progeny; F1-Δ rca-1 ; Δ cre-1, the rca-1 and cre-1 double deletion progeny; hph, hygromycin phosphotransferase gene; M, oligo-nucleotide marker.