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Kim, H.-J. & Benner, S. A. Prebiotic stereoselective synthesis of purine and noncanonical pyrimidine nucleotide from nucleobases and phosphorylated carbohydrates.
Another set of primers was designed to have an additional mismatch at the second nucleotide from their 3′ termini.
A salicyl alcohol as a cyclic bifunctional masking unit is used, and shown to afford a chemically driven release of the particular nucleotide from the lipophilic phosphate triester precursor molecule.
The distribution of the polymorphisms in our strain collection indicated the absence of a particular nucleotide from any of the SNP positions that was present in all AIEC strains and differed from the base found in that position in non-AIEC strains.
Normally, the biosynthesis of nucleoside antibiotics follows a distinct logic via sequential modifications of the simple building blocks including nucleoside and nucleotide from primary metabolisms (Isono, 1988).
To increase allele specificity, we gave an artificial mismatched nucleotide near the 3' end (second or third nucleotide from the 3' end) of the allele-specific primer (lowercase with underlined nucleotide in Figure).
A codon is defined by the initial nucleotide from which translation starts.
We have changed the second template nucleotide from C to G, preventing dGTP incorporation by simple slippage mechanism.
UMP is the pivotal nucleotide from which uridine nucleotides di-and triphosphates are formed by ATP-dependent kinases [3].
Cy3 was conjugated to a 3rd nucleotide from the end of 18 nt ssDNA [dT(15+3)] (Fig. 2B cartoon).
Therefore, we looked for inorganic phosphate-dependent (Pi-dependent) phosphorolysis of the 3'-terminal nucleotide from the primer DNA strand.
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