Sentence examples for nucleotide form from inspiring English sources
Then, orotate is converted to its nucleotide form in the presence of 5-phosphorylribose-pyrophosphate.
In MTAP-positive cells, abundant adenine, generated from supplied MTA, competitively blocks the conversion of an analog, by adenine phosphoribosyltransferase (APRT), to its active nucleotide form.
The three proteins from Drosophila head extracts discriminatively bound to either nucleotide form of Gαo in our experiment (Fig. 2B F), suggesting that the majority of Gαo target proteins interact equally well with GDP- and GTP-loaded Gαo.
We could identify three bands which bound preferentially to either nucleotide form of Gαo: two in the GTPγS-matrix (ca. 53 kDa and 71 kDa), and one in the GDP-matrix (ca. 37 kDa).
Sequences were translated prior to alignment to preserve codon structure and then returned to nucleotide form for analysis.
Most importantly, the motif invariably forms long-range tertiary contacts, as the AAA stack typically forms A-minor interactions and the flipped-out N nucleotide forms additional contacts that are specific to the structural context of each loop.
We have performed a proteomics analysis of the Drosophila proteins which would discriminate between the two nucleotide forms of Gαo, and revealed surprisingly few targets of this kind.
Though neither analog is activated by APRT, in MTAP-positive cells, adenine produced from supplied MTA blocks conversion of 5-FU and 6-TG to their toxic nucleotide forms by competing for 5-phosphoribosyl-1-pyrophosphate (PRPP).
HERC1, ubiquitously expressed, is located in the cytosol in the Golgi apparatus, stimulating guanine nucleotide, forming a cytosolic ternary complex with clathrin and Hsp70, and is involved in protein trafficking.
Through a combination of biophysical approaches, we now characterize the interactions between GoLoco1 and the two nucleotide forms of Gαo.
The CPT probe in contrast includes a modified RNA nucleotide forming a RNA-DNA duplex after hybridization to the target.
