Sentence examples for nucleotide extraction from inspiring English sources

Different protocols, including cell recovery, nucleotide extraction and purification, were compared on a panel of nucleoside mono-, di- and triphosphates from four cell lines (adherent and suspension cells).

The industrial production of both IMP and GMP is mainly achieved either by RNA breakdown and nucleotide extraction or by microbial fermentation using different microorganisms such as Corynebacterium, Bacillus, or Escherichia coli.

Nucleotide extraction and nucleotide cycling assay were performed as previously described [42].

Because direct measurement of cellular mADPR is confounded by the degradation of NAD and/or NADP into mADPR during nucleotide extraction procedures (reviewed in [25] [27]), our experimental approach utilized two indirect readouts of each mutant PARP's ability to support mADPR formation: NAD degradation and TRPM2-dependent cytosolic Ca2+ transients.

To prepare samples for nucleotide extraction, 0.5 grams of tissue was homogenized in 10 ml of Trizol LS per sample.

For example, "What is the use of RNAse in nucleotide extraction?" was used to assess specific knowledge gained, while "Why is cassava an important crop in this world?" was used to assess broad knowledge gained.

There was an improvement in all the questions, with GCQ7 ("What is the use of RNase in nucleotide extractions?") showing the highest increase of 155% and 177% during the first and second semesters, respectively.

Several versions of the P-postlabeling method were established, employing limiting concentrations of [γ-P]ATP to label DNA adducts selectively over nonmodified nucleotides, different conditions of enzymatic digestion of DNA, or pre-enrichment of DNA adducts from nonmodified 3′ nucleotides by extraction with n-butanol prior to postlabeling.

Moreover, some other tested conditions should be avoided, such as using pure methanol as extraction solvent (decrease over 50% of intracellular nucleotides extracted from NCI-H292 cells) or adding a purification step with chloroform.

Double stranded oligonucleotides were labeled using Klenow polymerase and [α-32P]dCTP and isolated from unincorporated nucleotides by gel extraction.

The cDNA mixture was then separated from unincorporated labelled nucleotides using nucleospin extraction columns supplied in the kit.