The second cDNA library was amplified by PCR with specially designed primer sets in which 6 nucleotides composed of CC (HaeIII-digested sequence) and 4 variable nucleotides were added to the 3′ end of the adaptor sequence.
TATA-binding protein (TBP) recognizes a characteristic sequence of nucleotides composed of deoxyadenosine and deoxythymidine (the TATA box) and binds to it, thus marking the starting point of transcription.
Aptamers are modified nucleotides composed of single-stranded nucleic acids that adopt a specific three-dimensional conformation, allowing them to bind with high specificity and affinity to molecular targets in a manner similar to that of monoclonal antibodies.
Each strand of this molecule is a series of nucleotides, and each nucleotide is composed of a sugar-phosphate compound attached to one of four nitrogen-containing bases.
Each nucleotide is composed of a phosphate attached to a ribose or deoxyribose sugar group which is attached to a nitrogenous base.
Each nucleotide is composed of a nitrogen-containing nucleobase either guanine (G), adenine (A), thymine (T), or cytosine (C as well as a monosaccharide sugar called deoxyribose and a phosphate group.
The B subregion at position 490 745 (numbers are from the first 5 S nucleotide) was composed of short 28-bp subrepeats.
Their Bayesian analysis of Hexanchiformes, in using only a small portion (15%) of a single nucleotide sequence composed of five genes, COX1, NADH2, CYTB, 16S, and Rag1, may be compromised by too little data.
The remaining sequence reads were compared with a nonredundant bacterial nucleotide database composed of the bacterial reference genomes and draft genomes deposited at the NCBI site (ftp://ftp.ncbi.nih.gov/genomes/, NCBI Refseq release 51, January 2012 and Genbank release 187, December 2011).
In our models, however, the switch loops mainly serve to anchor the lower half of the nucleotide cleft in place against the microtubule surface (via the closed hydrogen bond network, anchored by the N255 linchpin residue), while seesaw-like motion by the upper half of the nucleotide cleft (composed of the N-terminal subdomain) translates the P-loop down towards the switch loops.
