Sentence examples for nucleotide calling from inspiring English sources

Technical issues specific to 454 technology include: nucleotide calling difficulties within homopolymers; sequencing failure arising from incomplete homopolymer extension; base mis-incorporation by residual nucleotides during the nucleotide flow step; mixed template beads, and overrepresented single templates distributed across different beads [ 34].

When another half-sugar and half-base are added, the RNA nucleotide called ribocytidine phosphate emerges.

At the 5′ end of the mRNA, a modified guanine nucleotide, called a cap, is added.

Supported by funding from the National Institutes of Health, the Sondermann lab has focused specifically on a small nucleotide called cyclic diguanylate, or c-di-GMP, found uniquely within bacteria.

RNA is a very similar molecule to DNA except that the nucleotide thymine (T) is replaced by a nucleotide called uracil (U) and the sugar in the sugar phosphate backbone contains an additional oxygen molecule.

Nucleotide calls for these positions were obtained from the individual Illumina GA sequence reads.

Basically, polymerases pause at the template sequences where the limited nucleotides are supposed to be incorporated, and the temporal pausing of the polymerase triggers the incorporation of an incorrect nucleotide, called misincorporation, which eventually generates mutations.

Sanger sequencing confirmed 44 of these 54 to be identical to the nucleotide called by NGS.

At every base, any nucleotide called by a read is referred to as a "candidate base call".

Each octamer contains two known bases at the 3' end followed by three degenerate nucleotides and three nucleotides that can hybridize to any other nucleotide, called universal nucleotides.

However, these new nucleotides contain a different sugar (i.e., ribose) and instead of the T nucleotides use a fifth nucleotide called uracil (U).