Sentence examples for nucleotide accuracy from inspiring English sources

Nucleotide accuracy was assessed by comparing the overlaps between neighboring pools.

Our results clearly show that overall nucleotide accuracy approaches that of the Bermuda standard for accuracy of finished sequence—(i.e., 1 error in 10 kb).

The measure of prediction performance was taken in two ways: full exon accuracy and individual base (nucleotide) accuracy, according to the conventions of Burset and Guigó in [16].

Secondly, we assessed nucleotide accuracy by comparing those regions of O. barthii that lay in the overlapping regions between neighboring pools and were therefore sequenced twice.

If one assumes that for each position of disagreement between two pools, one is correct, then the overall nucleotide accuracy for the overlap regions is 99.978% or 2.2 errors for every 10 kb sequenced very close to the 1 in 10,000 Bermuda standard for accuracy of finished sequence (http://www.ornl.gov/sci/techresources/Human_Genome/research/bermuda.shtml).shtml

These methods reduce the amount of sequencing necessary, but do not provide single nucleotide accuracy.

However, aware of the higher nucleotide-accuracy of the Illumina technology compared to PacBio, we mapped the Illumina MiSeq contigs (not scaffolds) over the PacBio-generated C34-chromosome version 1.

Figure 2 Nucleotide level accuracy rate results with Markov order of 2, 5, and 8, respectively, for C. elegans, Chromosomes I V. Figure 3 Exon level accuracy rate results with Markov order of 2, 5, and 8, respectively, for C. elegans, Chromosomes I V. Figure 4 Nucleotide level accuracy rate results for three different kinds of settings.

Going beyond heuristics, emerging algorithms may enable automated design of RNAs with nucleotide-level accuracy but, as illustrated on a recent RNA square design, are not yet fully predictive.

This evolutionary technology demands a new algorithm to process sequence reads because of the different nature of its reads, whose nucleotide-level accuracy is only 85% [ 12].

Because the sequencing reaction in semiconductor-based NGSs does not use terminator nucleotides, the accuracy of the generated reads are known to decrease for homopolymer repeat sequences [ 10, 12, 17– 20].