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To determine the number of X chromosomes, non-overlapping nuclei were selected and the number of signals per nucleus was determined.
One hundred nuclei were selected randomly from each sample.
Metaphases and nuclei were selected with an AxioImager Z1 epifluorescence microscope (Carl Zeiss, Germany).
Areas of optimal tissue digestion and no overlapping nuclei were selected in each core for counting.
In each case at least 100 nuclei were selected, and 19 different geometric parameters were measured.
Nuclei were selected on the basis of colour and were expressed as a percentage of the total adenoma tissue in n=20 high power fields.
Similar(47)
A small area around each apparent cell nuclei was selected in all images and the mean fluorescence in arbitrary units (au) in these areas was calculated.
The orientation of recrystallized nuclei is selected between the orientation of the deformed grain and the orientation of the grain boundary area.
In order to overcome the complexity of the deformed microstructure, the orientation and location of the nuclei are selected by a set of proposed conditions based on texture and scatter in the measured orientation-dependent stored energies in the cold-rolled and stress-relieved states.
A focal plane that excluded the nuclei was selected for high-resolution imaging of the t-tubule structure.
This scenario could change however if some randomly activated prey genes in the host nucleus were selected and fixed in the population [47], [48] allowing them to survive within populations.
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