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Secondly, nuclei were defined as the objects of interest and their number was determined.
The subcellular localization of Stat1 was monitored by confocal microscopy; cell nuclei were defined using specific nuclear markers.
Myofibers with pale cytoplasm and loss of peripheral nuclei were defined as necrotic as previously described [30].
From DNA-staining images, cellular nuclei were defined.
Cells that had brown-yellow granules in the nuclei were defined as positive, whereas cells without brown-yellow granules in the nuclei were defined as negative [15].
Nuclei were defined in three different zones as recently described [ 23].
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Coronympha is found in several different termite species in the genus Incisitermes, but only two species of Coronympha have ever been described: C. clevelandi has 16 nuclei and is found only in I. immigrans [22], while any Coronympha with eight nuclei is defined as C. octonaria, and has been found in at least eight different species of Incisitermes [25], [28], [29].
As a result, the boundaries of nuclear landscape in hyperheavy nuclei are defined by spontaneous fission and not by the particle emission as in lower Z nuclei.
A positive nuclei was defined by a the red color with the TMR label and distinguished from autofluorescent background by a violet colored nuclei after merging the DAPI and Texas Red label.
Total TH-fiber area, excluding striatal nuclei, was defined using identical computer-assisted image thresholding.
The mean number of positive nuclei was defined as the apoptotic index.
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