As a first step, the DAPI-image is thresholded (static threshold manually set to an intensity value of 30) and turned into a binary image, with nuclei set to 0 and the remaining pixels set to 1. Next, the VE-c image is de-noised by using an itk-based median filter of radius set to 1 pixel.
Antoni A tissue is cellular and consists of monomorphic spindle-shaped Schwann cells, with a poorly defined eosinophilic cytoplasm and pointed basophilic nuclei, set in a variable collagenous stroma [30].
Neutrons strike the heavy uranium nucleus, which splits, releasing a tremendous jolt of energy along with two or more neutrons, which split more nuclei, setting off a chain reaction that grows and grows and finally manifests itself as a huge fireball over a populated area, blinding, asphyxiating, incinerating, or crushing every living being within a five-mile radius".
Double-tuned resonators are required for accurately co-registering multi-nuclei data sets, especially when the time courses of 1H and 23Na signals are of interest as, for instance, when investigating the pathological progression of ischaemic stroke tissue in vivo.
Nonepithelial nuclei are acquired by eliminating the identified epithelial nuclei N E from a set of nuclei N as follows: (2) N N E = N − N E. Table 1 summarizes the notations used in this paper.
Therefore, epithelial nuclei are identified by selecting the nearest nuclei to the lumen boundary, B O, from a set of nuclei N. A set of epithelial nuclei is denoted by N E and defined as (1) N E = ⋃ p ∈ B O { n | argmin n ∈ N (Distnace (Centroid (n ), p ) ) }, where p is a point in B O and the Centroid is a function returning the center point of a given object.
A set of nuclei called deep cerebellar nuclei is embedded in the white matter, away from the cerebellar surface (histological features of the cerebellum are annotated on the top-left micrograph in Figure 1).
Basic rules were set for nuclei to move in the VZ to avoid neighbouring nuclei: (1) A searching radius (SR) around each nucleus was assumed.
In both 3D images, it can readily be observed that the selected portion of the L-shaped set of nuclei exactly corresponds with the trace of the discovered furnace on the horizontal plane through its floor.
Early work on positional information in the Drosophila embryo was inspired by the idea of a morphogen gradient that is interpreted by the nuclei according to a set threshold (Ashe and Briscoe, 2006; Crick, 1970; Wolpert, 1969 , 1996.
The threshold was set to highlight nuclei and the "Analyze Particle" plug-in was used to count nuclei based on size.
