Sentence examples for nuclei removal from inspiring English sources

Accuracy of the micro-punches was confirmed with cresyl violet staining on the cryostat sections after nuclei removal.

In the eye, programmed removal of nuclei and other organelles from lens fiber cells is essential to remove light-scattering centers and provide an optically clear path to the retina.

The present data allow the first unified hypothesis for lens cell removal of nuclei [21], [22].

After removal of nuclei and cytosol, the luminal content of endomembranes was separated from the membrane fraction upon carbonate extraction as described in [16].

The eye lens presents a unique opportunity to explore roles for specific molecules in cell proliferation, differentiation and development because cells remain in place throughout life and, like red blood cells and keratinocytes, they go through the most extreme differentiation, including removal of nuclei and cessation of protein synthesis.

Briefly, after removal of nuclei, cell debris, cytosolic and microsomal cell fractions, the mitochondria pellet was resuspended in 0.5 M sucrose buffer (1 mM EDTA, 0.1% BSA, 10 mM Tris-HCl, pH 7.5), layered on a 1 2 M sucrose gradient (1 mM EDTA, 0.1% BSA, 10 mM Tris-HCl, pH 7.5) and centrifuged for 2 h at 25,000 rpm.

After removal of nuclei and debris by centrifugation at 1,000 ×  g for 5 min, the concentration of sucrose was adjusted to 40.6%.

After the removal of nuclei and non-disrupted cells, the lysate was incubated with nuclease S7 to destroy endogenous mRNA and to reduce background translation to a minimum [ 16].

These results reveal that MAPK/JNK is a positive regulator of MTORC1 signaling and its developmentally regulated inactivation provides an inducing signal for the coordinated autophagic removal of nuclei and organelles required for lens function.

Programmed removal of nuclei and other organelles from lens fiber cells occurs as elongating lens fiber cells detach from the capsule and later inter-digitate with apposing fibers to form sutures. Anoikis is a form of apoptosis resulting from loss of cell anchorage.

Determination of effective decellularization was based upon established criteria [41,42], specifically: (i) removal of nuclei as observed by imaging and analysis of H&E and/or DAPI stained sections; (ii) samples should possess <50 ng double stranded DNA (dsDNA) per mg initial dry weight.