Sentence examples for nuclei protein from inspiring English sources
Sections were then co-incubated for 48 hr at 4°C in rat anti-BrdU (1∶1000; Accurate Chemical), guinea pig anti-glial fibrillary acidic protein (GFAP) (1∶1000; Advanced Immunochemical), and mouse anti-neuronal nuclei protein (NeuN) (1∶1000; Chemicon).
At P8, ectopic GCs labeled by neuronal nuclei protein (NeuN) and residing in areas of highly disruptive basal lamina have stopped proliferating.
More than 15,000 peptides and 3,200 proteins were detected in 552,529 spectra obtained from whole cell and nuclei protein extracts.
Immunohistochemistry showed tightly compact bipolar cell nuclei (protein kinase C alpha/calbindin positive) with blur/loss of ON bipolar cell dendritic tips (transient receptor potential M1 positive) in diffusely condensed outer plexiform layer.
We stained by immunofluorescence mouse spinal cord cells with either anti-Pur-alpha or anti-FUS antibodies together with an antibody against Neuronal Nuclei protein NeuN, used as a neuronal marker.
Five days after viral injections, another subset of mice (two from each group) were killed to determine viral infection efficiency of the transplanted glioma cells, which were identified by staining for human nuclei protein.
Histones from Agave spp. clones (P20, P21, P159 and BM26) were isolated from 0.5 grams of leaf tissue from T0, M, B, SM and SB using sulfuric acid extraction of nuclei proteins followed by acetone precipitation, according to Jackson et al.[ 79].
DOI: http://dx.doi.org/10.7554/eLife.04251.002 Cell nuclei lack protein synthesis and therefore import required proteins from the cytoplasm.
This may be achieved, e.g., by placing two coding sequences inside the same loxP bracket in the Brainbow construct, one for the synaptic marker and one for the nuclei-bound protein, or by making expression of the synaptic marker trigger the expression of the respective nuclei-bound protein, etc.
Expression of microtubule associate protein-2 (MAP-2, green) and the neuronal nuclei-specific protein, neuron-specific nuclear protein (NeuN, red) demonstrated that >98% of the cells were indeed neurons.
As bacteria lack nuclei, bacterial proteins do not contain nuclear localizations signals (NLS), except in rare, presumably serendipitous, cases.
