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To study this position in greater detail, we reconstructed z stack movies of the nuclei of dividing cells and tracked nuclei position through division.
Based on the nuclei position in confocal analysis, the DEL cells were depleted from the leading edge region at 8 hpf (Figure 3B) relative to YSL and EVL nuclei.
Gray dashed line, initial nuclei position; black dashed line, skewed nuclei position; beige arrows, direction of cortical flow on the dorsal surface (Video 6 ).
Gray dashed line, initial nuclei position; black dashed line, skewed nuclei position; beige arrows, direction of cortical flow on the dorsal surface (ABa counter-rotating flow shown in Video 6 ).
As with Noc and LatB treatment, nuclei position in interphase cells was not affected by Myo10 knockdown.
By their morphology (roundish cells with prominent nuclei), position (middle third of the epithelium), and the weak levels of the Gα0-protein (arrowheads in Fig. 5b), they could correspond to maturing ORNs.
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Estimates of the fraction of early cells with either nuclei positioned at the bud neck or dividing nuclei were essentially zero, while estimates of early budded cells were around 1% (Table 2).
Instead, they became oval with centrally located nuclei in a nest-like structure, indicative of impairment in the nuclei positioning and/or cytoskeleton organization.
If the sum is taken over the oscillating electrons only (while the nuclei positions are fixed), ε ab is an electronic component of dielectric permittivity.
To include the bosonic degrees of freedom in the description, one can go beyond the Born-Oppenheimer approximation and expand the term V e N linearly in the nuclei positions.
Additionally, not all cells are flat nor are their nuclei positioned in the center of the cell.
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