It should be noted that nuclei pairs whose tracking ended in the VZ (61 of 76 cases; Fig. 5C and Fig. S3A, black bars, 248±140 min) were always engaged in apical-to-basal migration.
The main challenge with the binomial estimator is the identification of nuclei pairs.
The latter makes sense because some nuclei pairs that have recently divided remain connected by visible filaments.
Unlike wt, gck-1 lf) gck-1 lfith paired, thread-like chromosomes were nucleiranged in an orderly pattern on the gonad periphery, and there withno clear transition from pairedene into diplotene (usually just prior to the bend in wt gonad arms) (Figure 1chromosomes
We scored the frequency of nuclei with paired HIM-8 signals in five zones that correspond to the premeiotic stage and four substages of meiotic prophase.
In the premeiotic zone (zone 1), the frequency of nuclei with paired signals is less than 20% in both wild-type and pgl-1 mutant.
We experimented with several algorithms to automatically pair nuclei, primarily based on proximity.
Cap-H2 overexpression also led to a decrease in the proportion of nuclei with paired homologs from 80% of cells with paired homologs in control cells compared to 35% (P < 0.005) paired in Cap-H2-overexpressing cells.
Each pair of nuclei undergoes a mitotic division to form an eight-nucleate embryo-sac.
Noncentral potentials have been studied in various fields of nuclear physics and quantum chemistry, which may be used to the interactions between the deformed pair of nuclei and ring-shaped molecules such as benzene [1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12].
