Sentence examples for nuclei of dead from inspiring English sources
IrC1 specifically stained the nuclei of dead cells over living cells rapidly (<10 min) and at low concentrations (10 μM), as observed using confocal luminescence microscopy.
Propidium iodide (PI, abs: 510 560 nm, em: 600 650 nm) binds to DNA in the nuclei of dead cells, and is non-fluorescent when excluded from entering living cells by the intact cell membrane.
Moreover, cell death examined by TO-PRO3 dye exclusion test (non-cell-permeable dye and thus stains only nuclei of dead cells) did not show significant differences in the percentage of dead cells among hippocampal neurons transfected by GFP alone and PRR7-GFP (Supplementary Fig. 3c, d).
However, apoptotic cells had bright blue nuclei because of karyopyknosis and chromatin condensation; the nuclei of dead cells could not be stained [18 22].
When cells were treated with Hoechst 33,258, live cells with uniformly light blue nuclei were observed under fluorescence microscope, while apoptotic cells exhibited bright blue because of karyopyknosis and chromatin condensation, and the nuclei of dead cells could not be stained [30].
Recently, Li and Mombaerts [14] established mouse nuclear transfer embryonic stem (ntES) cells from embryos cloned from nuclei of dead cells after freezing without cryoprotectant.
After 15 min incubation at room temperature 1 µl PI was added to a final concentration of 50 µg/ml for red staining of nuclei in (dead or dying) cells having defective and permeable membranes.
Here, we used the membrane-impermeable propidium iodide staining for detection of nuclei in dead cells, which is dependent on a damaged plasma membrane for cell entry.
Images of 2.5 µm sections of whole islets were taken every 3 µm and the number of dead cells per islet were counted as nuclei stained with ethidium homodimer-1.
To quantify cell damage we calculated the extent of dead or damaged cells as defined using DAPI staining (which labels nuclei and shows enhanced fluorescence with dead or damaged cells) 24 h post transfection.
