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Secondly, we will compare classifiers with feature set extracted from epithelial nuclei objects.
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The process had four main steps: identification of nuclei (Primary objects), identification of nuclei plus cytoplasm (Secondary objects), determination of cytoplasm (Tertiary objects), and measurement of signals in tertiary objects.
Volume-object annotation system (VANO) is a cross-platform image annotation system that enables one to conveniently visualize and annotate 3D volume objects including nuclei and cells.
Once the nuclei were identified, the secondary objects were determined by dilating primary objects by 15 pixels.
Furthermore, users are able to customize the degree of background subtraction by applying a multiplier to each correction image, useful for identification of objects such as nuclei or cell borders.
Aberrant nuclei refer to nuclei with abnormal morphology, such as fragmented nuclei or nuclei with irregular shapes.
The implication is that the anterior thalamic nuclei and medial dorsal nuclei have subtly different roles concerning familiar objects.
The physician had to inspect segmented images against the original RGB images of each patient, with the task of identifying and excluding from the images segmented objects that were not nuclei.
Red are nuclei and green is target antibody.
For an siRNA to score as positive in the enhancer screen, it must cause a significant increase in the ratio of monopolar to interphase nuclei (color-coded as red and yellow objects in Figures 3 and 4) at 48 and 72 hour time points relative to the population average in a single experiment.
Nuclei were segmented using the 3D component analyzer plugin of the Fluorender (Voxelpress; http://www.voxelpress.com/); first, we counted nuclei-sized (mean ± 2SD) nlsLacZ-stained objects at low threshold, deleted the detected volumes, and then counted the remaining nuclei at higher threshold.
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