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Cell nuclei measure ca 10 μm in diameter, and contain nucleoli and, in a few cases, unravelled chromosomes (Fig. 4i, j).
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The fraction of nuclei at ploidy levels from 2C to 64C were expressed as a % of the total number of nuclei measured and the data were analyzed by hierarchical clustering with the Cluster and Treeview software [71].
A final score was determined by the percentage of brown stained nuclei (i.e. positive cells) over the total of tumor cell nuclei measured (i.e. positive plus negative cells).
We used Q-FISH in interphasic nuclei to measure the mean telomere length of 24-hpf larvae.
Using the measured antiproton flux and B/C ratio to fix the diffusion coefficient, we show that the spectra of primary nuclei as measured in the heliosphere may contain a fresh local "unprocessed" component at low energies perhaps associated with the Local Bubble, thus decreasing the measured secondary to primary nuclei ratio.
To determine if the low RFP signal detected in the yellow channel was uniform, we segmented nuclei and measured the ratio of yellow:red signals.
To assess the integrity of the cholinergic efferents from the laterodorsal tegmental nuclei, intensity measures were taken (following acetyl cholinergic staining) from the anteroventral thalamic nuclei and interpeduncular nucleus.
For each image captured, at least 3 different foci are analyzed for lesion and normal control (if available) respectively, with each focus containing >100 nuclei and measuring 0.1 to 1.0 mm in area.
The analysis module (Object Intensity 01) identified each cell by the blue staining of the nuclei, and measured both red (Cy5) and green (FITC) intensities in a 4 pixel ring around the nuclei.
To compare results from the computational model with in vivo results using the same criteria, we designed in silico experiments to label the S-phase nuclei and measure the distance the nuclei move after exiting S-phase.
Although the resolution of ICM-DNA histograms is still slightly lower than that of DNA histograms obtained by FCM-DNA, because of the lower number of nuclei that are measured in ICM-DNA analyses, the resolution in our study has been improved by measuring at least 2000 nuclei compared with measuring typically between 100 and 400 nuclei in the past (Brito et al, 1994).
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