Sentence examples for nuclei markers from inspiring English sources

We stained cells using β-III tubulin (Millipore Co., Billerica, MA, USA) as a marker for neuronal differentiation with a ratio of 1 500, nestin (Millipore Co., Billerica, MA, USA) as markers for motor neuron progenitor [10], and DAPI (Invitrogen, Carlsbad, CA, USA) as nuclei markers.

Regardless of the approach for whole cell staining, cells that are touching or partly overlapping can be automatically separated with the help of the nuclei markers of the first channel [2].

Immunofluorescence using an E-cadherin specific antibody was performed on 8 hpf embryos that were treated with ethanol or untreated (Fig. 4D,E), and compared to cell surface and nuclei markers (Fig. 4B K) in prechordal plate cells.

Thereafter, sections were incubated in DAPI (cell nuclei marker, 1∶1000, Invitrogen, USA), Alexa488-conjugated antibodies for mouse IgG and Alexa594-conjugated antibodies for rabbit IgG (1∶100, Invitrogen, USA).

However, none of the detected song nuclei marker genes are specific to finches.

For the few constructs generated in our laboratory, a more detailed analysis of their localization was performed using co-localization experiments with plasma membrane or nucleus markers (not shown).

The +/gcs1 plants were crossed with the transgenic Arabidopsis expressing HTR10-RFP (sperm nucleus marker), and the F1 +/gcs1 plants hemizygously expressing the marker were obtained.

When counter-stained with the nucleus marker 4',6-diamidino-2-phenylindole (DAPI), SCCs at the duct constitute approximately 20% of the total epithelial cells.

To observe the sperm behavior in an ovule, we produced a +/gcs1GAA line whose sperm cells express HTR10-RFP fusion as a sperm nucleus marker [13], in addition to the GFP signal from GAA (Figures 4A C).

Immunofluorescence study was performed using specific antibodies against βIII-tubulin (Tuj1), which labels neurons in early development as well as mature neurons [18], NeuN (neural nucleus marker), MAP2 (mature neuron marker), and glial fibrillary acidic protein (GFAP) to identify the differentiated level of astrocytic and neuronal populations.

(C ) Fluorescent overlay of ventral cilia (AcTub) with nucleus marker (DAPI) in indicated RNAi animals.