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The counterstaining with bisbenzimide for nuclei identification revealed an apparent normal distribution of perineurial nuclei, Schwann cell nuclei, and endothelial cell nuclei in the blood vessels).
Sections were stained with DAPI for nuclei identification.
DAPI for nuclei identification was excited in a separate exposure with a 405 nm laser and detected a 450/50 nm filter.
The cells were finally mounted in UltraCruz ™ mounting medium containing DAPI for nuclei identification (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA).
Brain areas that contained activated pixels were identified based on a stereotaxic atlas [55], i.e. images were overlaid with the neuroanatomical image for nuclei identification.
To handle such variations and make the contrast of the images consistent, we perform smoothing [ 43] and adaptive histogram equalization [ 44] prior to nuclei identification.
Similar(52)
A very small number of Kupffer cells appeared to have inclusions in the nuclei, but identification of cell types in unstained tissue is difficult and a more detailed study will be needed to confirm the identity of these cells.
The main challenge with the binomial estimator is the identification of nuclei pairs.
Identification of nuclei and foci as well as signal densitometry was performed by CellProfiler 2.0 software (Kamentsky et al, 2011).
The identification of nuclei spectra is an important step result for the subsequent quantitative analysis of the cell drug response for DCT treated and untreated MCF-7 cells.
The process had four main steps: identification of nuclei (Primary objects), identification of nuclei plus cytoplasm (Secondary objects), determination of cytoplasm (Tertiary objects), and measurement of signals in tertiary objects.
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