Sentence examples for nuclei fractionation from inspiring English sources

Moreover, nuclei fractionation of transfected cells reveals that ErbB1, either in the presence or in the absence of nucleolin, is not detected in the nucleus (Figure 3B), indicating that ErbB1/nucleolin interaction occurs mainly in the cell membrane.

Nuclei fractionation was performed as described previously with slight modifications.

This suspension represents the "shielded" nuclei fraction.

Biochemical fractionation of nuclei to localize nascent DNA, or visualization after labeling with short pulses of nucleotide analogues, has shown newly synthesized DNA and replication intermediates to be located at the NM and crucially not in the loops (Fig. 1).

We successfully purified nuclei and cytoplasm using cell fractionation and found that under basal conditions the majority of Bcl-3 is expressed in the cytoplasm compared with a low level of Bcl-3 in the nucleus.

The cell nuclear fractionation protocol was performed as previously published59.

Aberrant nuclei refer to nuclei with abnormal morphology, such as fragmented nuclei or nuclei with irregular shapes.

20 fractions were obtained after fractionation.

Nuclear and cytoplasmic fractionation was performed using an EZ nuclei isolation kit (Applygen Technologies Inc.; China).

The nuclei was washed twice with nuclei isolation buffer with Triton X-100 and once with nuclei isolation buffer only.

Results shown in Fig. 6A indicate the distribution of representative subcellular markers along a sucrose gradient (from 10% to 50% sucrose concentration), obtained upon fractionation of Luc-shRNA HK-2 nuclei-free cell extracts.