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Moreover, slight nuclei expression of GRO β was also reported in oral squamous cell carcinoma [ 22].
Thresholds were identified to distinguish between positive and negative nuclei expression for each protein using an image object information table.
Interestingly, Aravindan et al. [ 54] evaluated CXCR4 and CXCL12 immunoreactivity beside cytoplasm and observed, to a lesser extent, nuclei expression in tumour epithelial cells.
Interestingly, in Nuc1 rat, where a mutation in the Cryba1 gene inhibits normal programmed loss of nuclei, expression of both Bit1 and PKD remains abnormally high in mature lens fibers.
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At 40× magnification, nuclei showing expression of Ki-67 antigen in relation to all nuclei were counted.
BIRC5 immunostaining was mainly localized in cancer cell cytoplasm and nuclei, and OCT4 expression was localized in cancer cell nuclei (Additional file 3: Figure S1).
Alterations in the nuclei shape and expression of nuclear envelope-associated proteins were accompanied by altered distribution patterns of the repressive histone marks trimethylation on lysine 27 of histone H3, trimethylation on lysine 9 of histone H3, and heterochromatin protein 1-alpha in p63-null keratinocytes.
Reduction of Rb levels by shRNAi resulted in an increased proliferation index (number of nuclei), and increased expression of Mcm3 and Mcm5 (markers of DNA replication).
Most nuclei with endogenous expression of DUX4-FL showed a distinctly more punctate pattern of TDP-43 immunostaining than was observed in DUX4-FL-negative nuclei (Fig. 5).
TUNEL-positive nuclei, caspase-9 expression, or nuclear translocation of apoptosis inducing factor were not detected in respiratory-deficient neurons (not shown).
In our study, the improvement of functional parameters was associated with an acceleration of the regeneration phase, as demonstrated by higher amounts of positive Ki-67 nuclei, and increased expression of trophic factors in the kidney of MSC-treated mice.
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