However, two factors question if these ionic conditions correctly reproduce the environment of nuclei in vivo: the small-scale motion and conformation of chromatin in vivo are not reproduced in isolated nuclei, and experiments and theory suggest that small ions in the cytoplasm are not free in the soluble phase but are predominantly bound to macromolecules.
We used this gating strategy to determine thresholds for enumeration of GFP-positive nuclei in subsequent experiments and high throughoutput screening.
Patch clamp experiments on nuclei devoid of external nuclear membrane reveal the existence of channel activity compatible with KV10.1.
ChIP experiments, including nuclei purification, followed published protocols (Zhang et al. 2012).
Whole-sheet contraction was measured without nuclei labeling during blebbistatin experiments, and Microsoft Paint was used to quantify cell sheet diameters and subsequently, sheet areas.
Double fluorescence in situ hybridization and nuclei-specific gene inactivation experiments revealed that serotonin promotes bone mass accrual through Htr2c receptors expressed on the VMH nuclei, while appetite through Htr2b and Htr1a receptors expressed on ARC nuclei of the hypothalamus.
For all experiments examining intestinal nuclei number, visualization of intestinal nuclei was aided by rtIs14 [elt-2:: GFP; osm-10:: HT150Q]IV, which expresses an irrelevant neuron-specific transgene, osm-10:: HT150Q, in addition to an integrated elt-2 GFP elt-2 GFP
But two types of nuclei created in the Argonne experiments have revealed features that are inconsistent with the spherical shell model.
Sheep granulosa cells lyophilized according to the protocol described for mouse spermatozoa were used as nuclei donor in the first experiments [4].
Notwithstanding in our previous work we had observed the presence of NPs also in the mitochondria and in the nuclei [ 40], in these experiments NPs were confined only in cytoplasmic vesicles, even though, sometimes, the vesicle size was so enormous to modify nuclear shape and/or cause mechanical damages to the cell (see Figure 12).
