For focus information analysis, at least 65 nuclei for each condition were analyzed.
The images presented in the main figure are representative images of the 117-176 nuclevaluatedted per condition in three independent experiments.
For each of two experiments, fluorescence intensity from five random hair cells in each condition (nuclei and whole cells) was quantified using Adobe Photoshop.
Under normal conditions, atomic nuclei recoil when they emit gamma rays, and the wavelength of the emission varies with the amount of recoil.
The plasmodium may become very large, with millions of nuclei, but, ultimately, when conditions are right, it forms a series of small bumps, each of which becomes a small, fruiting body (a structure that bears the spores).
Under proliferative conditions, the nuclei count for the endothelial cell lines used increased up to 3-fold over the 7 day time period.
Under non-proliferative conditions, the nuclei count for cell lines (d-HMVEC and HBMEC) remained unchanged from day 1 to day 7, and the Ki67 expressing nuclei fraction ranged from 7 to 31%.
For example, results obtained at Fa = 0.75 indicate that the greatest differential effects were observed when using SN-38 and vincristine, where the drug dose required to achieve a 75% decrease in nuclei count under proliferation conditions were at least 100- and 90.9-times lower, respectively, than the drug dose required to achieve the same effect level under non-proliferative conditions.
The misorientation distribution of the nuclei and the deformed matrix governs the growth conditions of the nuclei and determines which parameters control growth of the nuclei.
Using a Nikon Diaphot microscope, the number of pyknotic nuclei were counted and expressed as a percentage of the total number of nuclei counted (n = >300 nuclei per condition).
Aberrant nuclei refer to nuclei with abnormal morphology, such as fragmented nuclei or nuclei with irregular shapes.
