For an siRNA to score as positive in the enhancer screen, it must cause a significant increase in the ratio of monopolar to interphase nuclei (color-coded as red and yellow objects in Figures 3 and 4) at 48 and 72 hour time points relative to the population average in a single experiment.
The possibility to use different nuclear targets opens the window for charm physics with nuclei or for color transparency studies, as well as for an intensive hypernuclear physics program.
Osteoclasts were identified by the presence of ≥ 3 nuclei and purple color.
(H – I′ ) The dorsal motor nucleus of the Xth nerve (blue and orange arrows), the motor nucleus of the XIIth nerve (green and red arrows), and axons from these nuclei (arrowheads, same color scheme) in consecutive coronal (H a d′) and sagittal (I and I′ ) sections.
Histomorphological staining was performed as previously described [ 45]: slides were deparaffinized, rehydrated, stained with Safranin O (which colors proteoglycans red), counterstained with Fast Green FCF (which colors proteins green) and with Weigert's hematoxylin (which colors nuclei black), dehydrated, cleared, and mounted in Permount.
However, we do know that hematoxylin is a basic stain that colors nuclei and ribosomes blue/purple, and eosin is an acidic stain that colors protein-rich cytoplasm and extracellular matrix pink/red.
The H&E staining method colors nuclei of cells blue by Hematoxylin, and the nuclear staining is followed by counter-staining with Eosin, which colors other structures in various shades of red and pink.
Cells treated with etoposide were significantly accumulated at S/G2/M phases with nuclei labeled with green color, consistent with the pharmacological action of etoposide that inhibits topoisomerase II, thereby arrests the cells at S and G2 phases.
However, no mitotic cells were observed in etoposide-treated synchronized cells, total number of the cells gradually decreased, and a large fraction of the cells had nuclei labeling with green color (Supplementary Movie S6), suggesting that etoposide-treated cells arrested at S and/or G2 phases.
Coloring of nuclei corresponds to gene expression intensities.
We used DAPI as a nuclear label so as to achieve sufficient color separation between nuclei and PGHS-1, which is a cytosolic enzyme.
