Sentence examples for nuclei assays from inspiring English sources

CS and MSC participated in performing and designing the pyknotic nuclei assays.

The percentage of apoptotic and dividing nuclei (assayed by staining with the fluorescent nuclear probe DAPI) present during the differentiation period (0-12 dare) areportedted in Table 1.

In fact, the percentage of hypodiploid nuclei, as assayed by propidium iodide staining, ranged from 50% for MOLT-3 and HT-29 cells to 20% for SH-SY5Y cells following treatment with 100  μM 𝒟lentiginosine (data not shown).

PGi nucleus was assayed for the expression of BDNF and NT-3 using semi-quantitative RT-PCR normalized to β-actin gene expression.

Levels of the active/phospho-NF-κB p50 in the nucleus were assayed using TransAM NF-κB p50 Transcription Factor Assay Kit (Active Motif) according to manufacturer's protocol.

Relocation of the yeast cytoplasmic linear plasmids into the nucleus was assayed using a monitor plasmid pCLU1 in terms of the phenotypic change of the integrated markers (the UCS-fused LEU2 and the native URA3) in S. cerevisiae (leu2 ura3).

NMD substrates classified based on cell fractionation assays as "nucleus associated" or "cytoplasmic" are all inhibited in the same manner.

Intriguingly, RNA polymerase II transcription appears to be reduced in dauer larvae relative to other stages based on run-on transcription assays with isolated nuclei (Dalley and Golomb 1992).

The results of the traditional karyotyping and of FISH and M-FISH assays on metaphase nuclei reported in this study highlight that complex structural alterations of Chr17 encompassing the HER2 gene and CEP17 are common in breast cancer cell lines.

This could be done by experiments testing either whether Ssa2 shuttles between the cytoplasm and the nucleus, whether Ssa2 enters the nucleus in the absence of Ran, whether a Ssa2/tRNA complex binds FG nucleoporins or even whether Ssa2 alone or in complex with tRNA translocates to the nucleus in in vitro transport assays (using mammalian cells).

For each sample, two images of DAPI and two corresponding images of γH2AX were recorded on a Leica Epi-fluorescence Microscope using Image Spot Advance software and analysed by ImageJ and a custom macro, PZFociEZ (http://www.pzfociez.com/), to count the number of foci/nucleus in at least 100 nuclei per assay.