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Briefly, DAPI-stained nuclei and GFP-transfected cells were identified using Otsu's method, nuclei areas were subtracted from the identified cells to give a cytoplasmic GFP intensity and this was used to give a nuclear/cytoplasmic intensity ratio for each image.
We developed the ImmunoRatio image analysis software, which segments the DAB- and hematoxylin-stained nuclei areas from a microscope image, calculates the labeling index (percent of DAB-stained area out of the total nuclear area), and generates a pseudo-colored result image matching the segmentation.
In contrast, muscle sections from Mbnl1 ΔE3/ΔE3/mice3 ΔE2 mice demonstrate some centralized nuclei, areas of very small atrophied fibers and regions of potential fibrosis (Fig. 2F).
Degenerative changes, characterized by interrupted continuity of hypertrophic layer, pyknotic and eosinophilic lesion with few nuclei, areas filled with eosinophilic nuclei, were observed in more joints from female experimental groups than male ones.
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The cell number, defined by count of DAPI stained nuclei on membranes, as well as the measured nuclei area, were not influenced by the application of DON from apical side at any concentration or time in comparison to the control (DON0).
Signal quantification was calculated for each nucleus by determining the nuclei area and quantization of the signal in that area.
A 2D scatter plot showing combined nuclei area on the Y axis, vs cell area on the X axis, for control (green) and Ki-67 depleted (red) cells.
Ten features were calculated from the morphology of the nuclei (area, perimeter, eccentricity, length major axis, length of minor axis, convex area, solidity, equivalent diameter, rectangularity, and compactness).
The NC ratio of the volunteer was then obtained by the total summation of nuclei area from all chosen frames divided by that of cytoplasm area.
(C ) The two orange and two blue arrows show the phenotypes (nuclei area and cell number) of the two dsRNAs designed for sti and Cdc23.
To calculate the distance of the detected telomeres in relation to the nucleus, the single z-stack image with the largest nuclei area was analyzed.
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